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Image Search Results
Journal: Oncotarget
Article Title: Identification of the histone lysine demethylase KDM4A/JMJD2A as a novel epigenetic target in M1 macrophage polarization induced by oxidized LDL
doi: 10.18632/oncotarget.17748
Figure Lengend Snippet: ( A ) RAW264.7 cells were exposed for 1 hr to 50 μg/ml oxLDL with or without pre-treatment with the JMJD inhibitor JIB-04 for 4 hrs, after which Western blot analysis was carried out to monitor expression of the indicated proteins. ( B ) RAW264.7 cells transfected with shKDM4A and scramble control shRNA were exposed to 50 μg/ml oxLDL for 1 hr, after which expression of HIF-1α, -1β, and phosphorylated p65 (S536) was monitored by Western blot analysis. ( C ) RAW264.7 cells were exposed for 1 hr to 50 μg/ml oxLDL with or without pre-treatment with the IKK inhibitor parthenolide (PTL) for 2 hrs, after which expression of phosphorylated p65 and KDM4A was assessed by Western blot analysis. ( D ) RAW264.7 cells were transiently transfected with pGreenpuro-shRNA constructs specifically targeting HIF-1β (shHIF-1β-1 and -2) or scrambled sequence as control. Down-regulation of HIF-1β was validated by Western blot analysis (upper panel). Cells were then exposed to 50 μg/ml oxLDL for 1 hr, after which expression of KDM4A was monitored by Western blot analysis.
Article Snippet: The
Techniques: Western Blot, Expressing, Transfection, Control, shRNA, Construct, Sequencing